The skin is the largest organ of the human body and represents our first line of defense against external factors. Its diverse functions include the protection from pathogens, thermal insulation, temperature regulation, as well as sensation and wound healing. This functional diversity is achieved by a complex interplay between different cell types present in different skin layers. Located above the dermis (connective tissue) and the hypodermis (subcutaneous tissue), the epidermis forms the outermost layer of the skin and maintains homeostasis against physical, chemical and biological stresses.
Keratinocytes represent the main cell type found in the epidermis and are thus attractive tools for studying wound healing and stress responses, as well as sensitizing properties and overall toxicity of test compounds. To increase your throughput, we offer standardized, ready-to-use upcyte® Human Epidermal Keratinocytes isolated from juvenile donor material. For the culture of primary and upcyte Keratinocytes, we have developed a new chemically defined and animal component-free defined Keratinocyte Culture Medium.
Migration of upcyte® keratinocytes was determined by a wound healing assay. Confluent monolayers of upcyte® keratinocytes were scratched using a pipette tip. Wound closure was achieved after 8 h.
upcyte® keratinocytes were labeled with specific antibodies directed against cytokeratins 5/14, 8 and 1/10. Cells were counterstained using DAPI and analyzed by fluorescence microscopy.
More interested in primary keratinocytes? We also still have primary cells from our upcyted donor available (Donor 237) .The cells show a good proliferation capacity in the defined Keratinocyte Culture Medium, i.e.the cells are capable of up to 15 population doublings.
For each product a protocol for use and a certificate of analysis will be delivered. All cells have been tested for the absence of CMV, HIV, Hepatitis B and C and microbial contaminants (fungi, bacteria and mycoplasma). upcyte® cells are genetically modified cells (GMO, genetically modified organism) classified as risk group 1 and have to be handled in a genetic engineering facility carrying out genetic engineering work at Security Level 1 (e.g. S1 lab). upcyte®Hepatocytes and upcyte® Endothelial Cells are officially classified as risk group 1 organisms by the German Federal Office of Consumer Protection and Food Safety (BVL) and the Central Committee on Biological Safety (ZKBS). For further information please use the following link and enter “upcyte” in the search box.