upcyte® hepatocytes
expanded primary cells

The liver is the main metabolic organ responsible for pathways such as glycolysis, glycogenesis, ureagenesis, as well as amino acid and lipid metabolism. The predominant cells type is the hepatocyte; therefore, cultured human hepatocytes are suited for in vitro metabolism studies. upcyte® Hepatocytes are expanded primary cells, derived from single donor primary cells. We can expand up to 2000 vials per hepatocyte donor from one vial starting material – data from different studies can now be compared while working with the same donor.

applications
  • metabolism (CYP induction & inhibition, transporters)
  • various toxicity formats
  • HCV infection and virus replication
  • various 3D formats & co-cultures
  • transfection studies
Benefits of upcyte® hepatocytes at a glance
  • different donors available
  • batch sizes up to 3000 vials per donor
  • primary cell morphology & polarity
  • proliferating & metabolic competent
  • phase I, II & III activities
  • transfectable
  • long term cultures for up to 4 weeks
  • characterized, standardized, cryopreserved, assay-ready
upcyte® hepatocytes have been tested at each expansion state for
  • cell morphology
  • viable recovery (>70%) and high plateability (>90%)
  • markers: CK8+, CK18+; HSA+, AAT+, AFP-
  • capacity for glycogen storage (PAS staining)
  • basal and inducible CYP activities (Phase I)
  • urea secretion (basal and stimulated)
Need more info & data?

Please see our download center with posters and publications.

upcyte® hepatocytes are provided in 2 ml cryo-vials with a minimum of 5×106 frozen cells per vial and are ready-to-use. Our cryopreserved upcyte® hepatocytes are at a later state in proliferation terms. Further expansion will lead to the cells expressing senescence genes and the phenotype is lost.

To choose the most suitable donor (LOT#) for your specific application, please write an email to info@upcyte.com. Currently we have 6 different donors available (#732-03, #653-03 (+#653-2D6), #568-03, #151-03, #10-03, #422a-03 and #740 for genotox). If you need any help – be it theoretical or practical – our specialists are happy to help and demonstrate in your lab the handling of upcyte® hepatocytes.