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Media

All our Media are optimized for use with upcyte® cells and other primary cells.

We have developed a variety of media for the expansion, culture and endpoint measurement of the upcyte® cells. For the usage of our upcyte® cells in different applications, we recommend the use of the cell-specific media. A protocol for use will be included in each delivery.

 

upcyte-media-flaschen022Hepatocyte Thawing Medium (50 mL)

Use this medium to thaw cryopreserved human upcyte® Hepatocytes. It is supplemented with 10% FBS.

Hepatocyte Culture Medium (500 mL)

Hepatocyte Culture Medium is a serum free medium especially designed for the optimal subculture of human upcyte® Hepatocytes. This medium is suitable for the expansion of human upcyte® Hepatocytes from a master cell bank to a working cell bank stage under a license.

Hepatocyte High Performance Medium (500 mL)

Hepatocyte High Performance Medium is a serum free medium especially designed for the optimal short time culture e.g. 3D assays and transporter function. It consists of basal medium and one supplement, which has to be added to the basal medium prior to use. The medium can also be applied for endpoint determination of human primary hepatocytes.

Supplement E for Hepatocyte Genotox Assay

Supplement E is added to the Hepatocyte Culture Medium for an optimized in vitro MN genotoxicity assay.

 

LSEC Culture Medium (100 mL & 500 mL)

The LSEC Culture Medium is especially designed for use in endpoint experiments. It consists of basal medium and one supplement, which has to be added to the basal medium prior to use.

 

Airway Epithelial Cell Culture Medium (500 mL)

This Medium is especially designed for use with the upcyte® Bronchial Epithelial Cells. It consists of basal medium, L-Glutamine and three supplements, which have to be added to the basal medium prior to use.

 

Keratinocyte Culture Medium (500 mL)

This Medium is especially designed for use with primary keratonocytes. It consists of basal medium, L-Glutamine and three supplements, which have to be added to the basal medium prior to use.

 

Natural Killer Cell Culture Medium (100 mL)

This Natural Killer (NK) Culture Medium is designed for the optimal culture and expansion of primary human NK cells. The Medium is "animal derived component free" (ADCF) and contains cell type specific growth factors. Use of this NK Cell Culture Medium allows the induction of NK cell proliferation with an expansion rate of over 50-fold. Please note that the performance may vary as NK cell expansion is donor-dependent. It consists of basal medium and four supplements, which have to be added to the basal medium prior to use.

 

Smooth Muscle Cell (SMC) Culture & High Performance Medium (500 mL)

SMC Culture Medium: This Medium is especially designed for optimal growth of primary SMCs and promotes the optimal proliferative capacity of SMCs. Cells grown in this medium form less contractile structures and are positive for smooth muscle α-actin and negative for calponin. It consists of basal medium, FBS, L-Glutamine and two supplements, which have to be added to the basal medium prior to use.

SMC High Performance Medium: This Medium is especially designed for a controlled induction of differentiation of SMCs into a contractile phenotype. A cultivation period of 5 days is necessary for SMCs to form myofibril structures. By immunostaining for α-actin and calponin myofibril structures can be shown. It consists of basal medium, L-Glutamine and two supplements, which have to be added to the basal medium prior to use.

 

Skeletal Muscle Cell (SkMC) Culture & High Performance Medium (500 mL)

This medium conform to the same principle as the SMC Medium and is designed for primary human SkMCs.

 

Endothelial Cell Culture Medium (500m L)

This Medium is especially designed for use with primary endothelial cells. Endothelial cells cultured in this Medium retain primary cell characteristics over the course of the expansion. The specific markers, CD31 and van Willebrand factor, were clearly expressed and over 90% of the endothelial cells are negative for senescence markers. It consists of basal medium, L-Glutamine and three supplements, which have to be added to the basal medium prior to use.

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