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upcyte® Skeletal Muscle Cells (SkMCs)

Skeletal muscle cells are responsible for all voluntary body movements which are established by contractions of the skeletal muscles. An impaired skeletal muscle function is therefore involved in the pathology of many disorders of the locomotor system such as:

  • muscular dystrophy
  • muscle atrophy/hypertrophy
  • musculoskeletal disorders (MSDs)

upcyte® SkMCs feature an extended proliferation capacity whilst maintaining primary cell characteristics and morphology - ideally suited to study biochemical and molecular mechanisms of locomotor diseases. Large batches of quality-controlled, characterized upcyte® SkMCs from a single donor can be provided along with the corresponding media.

 

upcyte® SkMCs exhibit typical primary features of skeletal muscle cells

upcyte® SkMC have an uniformly population doubling with a population doubling time of ~32 h in Skeletal Muscle Culture Medium. Under these culture conditions, the cells exhibit a compact, spindle-shaped morphology and are positive for desmin as well as slightly positive for Myosin Heavy Chain 2.

SkMC CM

 upcyte® SkMCs differentiate in Skeletal Muscle Cell High Performance Medium

When grown in Skeletal Muscle Cell High Performance Medium, upcyte® SkMCs fuse into myotubes within 3 days and form syncythia. These cells are positive for desmin & myosin heavy chain 2 as well as sarcomeric structures become visible.

SkMC HPM

 

Functional test: atrophy and hypertrophy can be induced in upcyte® SkMCs

Atrophy of upcyte® SkMCs can be induced with 50µM Dexamethasone and Myostatin (data not shown), as established by quantification of MHC-2 immunofluorescence in upcyte® SkMCs. The atrophic effect can be reversed by inhibition with IGF-1 (1µg/ml). Hypertrophy of upcyte® SkMC can be induced with IGF-1 (1µg/ml). The hypertrophic effect can be reversed by inhibition with 10µM LY294000 (data not shown). Contact us if you want to hear more (Diese E-Mail-Adresse ist vor Spambots geschützt! Zur Anzeige muss JavaScript eingeschaltet sein!).

 

For each product a protocol for use and a certificate of analysis will be delivered. All cells have been tested for the absence of CMV, HIV, Hepatitis B and C and microbial contaminants (fungi, bacteria and mycoplasma). upcyte® cells are genetically modified cells (GMO, genetically modified organism) classified as risk group 1 and have to be handled in a genetic engineering facility carrying out genetic engineering work at Security Level 1 (e.g. S1 lab). upcyte® Hepatocytes and upcyte® Endothelial Cells are officially classified as risk group 1 organisms by the German Federal Office of Consumer Protection and Food Safety (BVL) and the Central Committee on Biological Safety (ZKBS). For further information please use the following link and enter “upcyte” in the search boxlink.

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