Bronchial epithelia lining the airway surfaces are involved in the immune response to airborne allergens and pathogens as well as in the defense against pollutants. Bronchial epithelial cells are therefore an important tool to study biochemical and molecular mechanisms underlying diseases like
• Cystic Fibrosis (CF), or
• Chronic Obstructive Pulmonary Disease (COPD).
upcyte® BEpCs feature an extended proliferation capacity whilst maintaining primary cell characteristics and morphology - ideally suited to study the cell biology of bronchial diseases. Large batches of quality-controlled, characterized upcyte® BEpCs from a single donor can be provided along with the corresponding medium.
upcyte® BEpCs exhibit an extended proliferation capacity up to 35 population doublings compared to primary bronchial epithelial cells. The cells exhibit the typical compact palisade morphology of primary epithelial cells and are positive for cytokeratin 8, 5/14 & 18 and negative for Ck1/10.
Functional test: upcyte® BEpCs respond to pollutant
The expression of MMP-1 (matrix metalloproteinase 1) in upcyte® BEpCs could be induced by 5 nM of the environmental pollutant TCDD (2,3,7,8-Tetra-chlorodibenzo-p-dioxin) in the culture medium. TCDD incubation resulted in a more than two-fold increase in MMP-1 concentration in the culture medium:
For optimal performance, upcyte® BEpCs should be cultured in our Airway Epithelial Cell Culture Medium.
For each product a protocol for use and a certificate of analysis will be delivered. All cells have been tested for the absence of CMV, HIV, Hepatitis B and C and microbial contaminants (fungi, bacteria and mycoplasma). upcyte® cells are genetically modified cells (GMO, genetically modified organism) classified as risk group 1 and have to be handled in a genetic engineering facility carrying out genetic engineering work at Security Level 1 (e.g. S1 lab). upcyte®Hepatocytes and upcyte® Endothelial Cells are officially classified as risk group 1 organisms by the German Federal Office of Consumer Protection and Food Safety (BVL) and the Central Committee on Biological Safety (ZKBS). For further information please use the following link and enter “upcyte” in the search box.